Journal: Journal of Orthopaedic Translation

Article Title: ADSC-derived mitochondrial nanovesicles transplantation alleviates capsular fibrosis and inflammation and improves joint mobility in a rat model of adhesive capsulitis

doi: 10.1016/j.jot.2026.101195

Figure Lengend Snippet: Single-cell transcriptomic analysis and immunohistochemical analysis of joint capsule tissues from normal individuals and patients with adhesive capsulitis. (A) UMAP visualization of all cells. (B) Oxidative phosphorylation scores of subpopulations. (C) GSEA of differentially expressed genes in fibroblasts. (D) UMAP visualization of fibroblasts. (E) Oxidative phosphorylation scores of fibroblast subpopulations. (F) Representative images of IHC staining and the semi-quantitative statistical analysis for Col I, Col III, Cox4, Opa1, IL1 and TNF a. Statistical significance was determined by two-tailed two sample t test. *P < 0.05 , **P < 0.01 , ***P < 0.001, **** P < 0.0001; n = 4–5.

Article Snippet: Primary antibodies against Collagen type I (Col1, Cat. No.: A24112), Collagen type III (Col3, Cat. No.: A3795), matrix metalloproteinase 9 (MMP9, Cat. No.: A25299), matrix metalloproteinase 13 (MMP13, Cat. No.: A11755), IL-6 (Cat. No.: A0286), IL-10 (Cat. No.: A2171) and tumor necrosis factor-alpha (TNF-α, Cat. No.: A21265) (all Abclonal, 1:300 dilution) were applied at 4 °C overnight.

Techniques: Single Cell, Immunohistochemical staining, Adhesive, Phospho-proteomics, Immunohistochemistry, Two Tailed Test

Journal: Journal of Orthopaedic Translation

Article Title: ADSC-derived mitochondrial nanovesicles transplantation alleviates capsular fibrosis and inflammation and improves joint mobility in a rat model of adhesive capsulitis

doi: 10.1016/j.jot.2026.101195

Figure Lengend Snippet: Effects of AD-Mito-NPs on migration and ECM-related protein expression in joint capsule fibroblasts. (A) Representative images and quantification of cell migration assessed by wound healing assay. Scale bar, 100 μm. n = 4. (B–F) Representative immunofluorescence images (left) and quantification of fluorescence intensity (right) for collagen I (Col1, B), collagen III (Col3, C), fibronectin 1 (FN1, D), matrix metalloproteinase 9 (MMP9, E), and matrix metalloproteinase 13 (MMP13, F). Red: target protein; Green: Phalloidin (F-actin); Blue: DAPI (nuclei). n = 3. Scale bar, 50 μm. (G) Schematic diagram illustrating phenotypic changes in fibroblasts following inflammatory stimulation and AD-Mito-NPs delivery. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA (B–F) or two-way ANOVA (A) with Tukey's multiple comparisons test. *P < 0.05 , **P < 0.01 , ***P < 0.001, **** P< 0.0001.

Article Snippet: Primary antibodies against Collagen type I (Col1, Cat. No.: A24112), Collagen type III (Col3, Cat. No.: A3795), matrix metalloproteinase 9 (MMP9, Cat. No.: A25299), matrix metalloproteinase 13 (MMP13, Cat. No.: A11755), IL-6 (Cat. No.: A0286), IL-10 (Cat. No.: A2171) and tumor necrosis factor-alpha (TNF-α, Cat. No.: A21265) (all Abclonal, 1:300 dilution) were applied at 4 °C overnight.

Techniques: Migration, Expressing, Wound Healing Assay, Immunofluorescence, Fluorescence

Journal: Journal of Orthopaedic Translation

Article Title: ADSC-derived mitochondrial nanovesicles transplantation alleviates capsular fibrosis and inflammation and improves joint mobility in a rat model of adhesive capsulitis

doi: 10.1016/j.jot.2026.101195

Figure Lengend Snippet: IHC analysis of adhesive capsulitis tissues in rats treated with AD-Mito-NPs or Dex and serum biochemical indicators analysis. (A-B) Histological evaluation of collagen deposition and organization in the joint capsule. (A) Representative images of Masson's trichrome staining, picrosirius red staining, and IHC staining for Col I and Col III. Scale bar, 100 μm. (B) Semi-quantitative statistical analysis of collagen content and the Col1/Col3 ratio based on IHC and histological stains. (C-D) Assessment of inflammatory infiltration. (C) Representative IHC images of pro-inflammatory cytokines IL-6 and TNF-α. Scale bar, 100 μm. (D) Semi-quantitative statistical analysis of positive area based on IHC staining. (E-F) Analysis of matrix metalloproteinases (MMPs) expression. (E) Representative IHC images of MMP9 and MMP13. Scale bar, 100 μm. (F) Semi-quantitative statistical analysis of positive area based on IHC staining. (G) Quantitative analysis of key serum biochemical indicators. n = 6. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA (B, D, F and G) with Tukey's multiple comparisons test. *P < 0.05 , **P < 0.01 , ***P < 0.001, **** P < 0.0001.

Article Snippet: Primary antibodies against Collagen type I (Col1, Cat. No.: A24112), Collagen type III (Col3, Cat. No.: A3795), matrix metalloproteinase 9 (MMP9, Cat. No.: A25299), matrix metalloproteinase 13 (MMP13, Cat. No.: A11755), IL-6 (Cat. No.: A0286), IL-10 (Cat. No.: A2171) and tumor necrosis factor-alpha (TNF-α, Cat. No.: A21265) (all Abclonal, 1:300 dilution) were applied at 4 °C overnight.

Techniques: Adhesive, Staining, Immunohistochemistry, Expressing